When Sexing Technologies, the big bull studs, or even Trans Ova produces sexed semen, the post-thaw motility standards are supposed to be the same as conventional semen. 50% motility at immediate post-thaw and 30% at three hours. Some bulls are higher than others, but thats the same old story for all bulls with any semen.
When you look at a straw of conventional semen post-thaw, you've still got to account for all the primaries and secondaries. There is plenty of data to show that those cells never make it to the egg. During the sorting process, most cells with primaries and secondaries are not included in to a straw of sexed semen. The computer selects each individual cell before it is packed in to the straw, so the quality of a sexed straw is actually higher than a conventional straw. Its just that in a straw of conventional, there are more total cells, whether good or not. Thats why some bulls sort better than others. Some bulls are genetically predisposed to producing more morphology problems. YES...when you perform a flush with sexed semen, use the 5.0 million dose concentration not the 2.1 million concentration!!!
When you compare IVF to flushing, there are some major differences. When the recipe is right with IVF; i.e. the right bull and cow, the results are awesome. Some bulls work well to AI with, but don't work well in an IVF situation and vice versa. Thats just a mystery of mother nature that we can't explain. Thats why its a good idea to have a couple of bulls to work with when you're performing IVF.
Where IVF gets really good is over time. Over multiple IVF sessions, you can make so many more embryos than you can through a flush. Plus, you don't have the super ovulation drugs involved and you can aspirate females as young as 9 months of age. When you add sexed semen to the equation, you can really spice things up. There also seems to be big differences in pricing for IVF from center to center. I 've noticed that some centers still charge up front costs no matter if embryos are produced or not. Our guy only charges on a per embryo produced basis. So when we bring in a donor, if she doesn't produce any embryos, then there's no charge. Its a pretty fair pricing system, because he has to produce good results to get paid.