HOW TO SUBMIT SAMPLES FOR THE GENE HUNT
IN A PERFECT WORLD YOU WOULD SUBMIT SAMPLES FROM CALF, SIRE AND DAM BUT IF YOU ONLY HAVE THE CALF THAT IS OK - YOU SHOULD STILL SUBMIT A SAMPLE FROM THE CALF
ALSO NOTE - while hair samples are adequate for many things in adult cattle they are less than ideal for gene hunts especially if the sample is from a calf - if you can only get hair you will need more hair from a calf - ie maybe 50 strands of tail hair with the bulb attached (the bulb is where the DNA is) - BLOOD is better from a live calf
[size=10pt]
- affected calf - if dead, take a chunk of ear (1 x 1 inch is enough) stick it in a baggy, label it with a sharpie (calf number, sire & dam, farm name) - put in refrigerator (or if you can't send it right away you can freeze it)
-affected calf - if alive - blood sample in purple top tube (make sure you rock the tube back and forth as it has an anti coagulant and you don't want the blood to clot - the DNA is in the nucleus of the white cells) or you can get a tissue sample from the ear using a pig ear notcher
- dam of calf - purple top tube - label with dams name, breed and reg # if registered
- sire - if he is yours and has not been collected - purple top tube; if he is yours and has been collected send a straw of semen (it can thaw); if he is an AI sire most likely DNA will be on file; label with name, breed and reg # if registered
-pictures of affected calf, description of abnormality by you or vet, and if calf was necropsied copy of necropsy report; short video if you think that would help
- pedigree of affected calf
-your name, address and phone number (and email)
- send samples on Monday or Tues (depending on where you live) - don't want them sitting somewhere over the weekend; if they can get to Illinois from your place ground in 2 days that is ok, otherwise send 2nd day; unless you are sure it will stay cold include an ice pack. If you have a small styrofoam container that is good - also make sure everything is capped tight, tubes padded, and put everything in a zip lock baggie
[size=10pt]- samples for a gene hunt go to Dr Beever (address below)
Jonathan E. Beever, Ph.D.
Department of Animal Sciences
University of Illinois at Urbana-Champaign
220 Edward R. Madigan Laboratory, MC-051
1201 West Gregory Drive
Urbana, IL 61801 USA
Some associations will pay for a complete necropsy on calves with abnormalities - generally for those abnormalities that are not confined to a limb (ie TH, PHA, AM, NH etc - the really gross ugly ones) - Dr Steffen in Nebraska generally does them and calves are sent to him (sometimes frozen) - sometimes a closer vet pathologist will work with the association and Steffen to do the necropsy - I always suggest that people submit samples before the calf goes away for necropsy and that you keep a chunk of ear in your freezer - that way you know (a) the sample got the the gene gurus and (b) you always have a sample
Most breed associations have a genetic defect policy - ie how you report abnormal calves - generally it is not obvious when you go to their web site and often it is placed in some obscure place -
If you have a calf with an abnormality and you live near a U or state vet lab you can always take the calf for necropsy, in some instances you can arrange to ship it for necropsy
Samples from Canadian calves with abnormalities can be shipped with an import permit that Dr B has
[/size][/size]