sexed semen timing

[bob b]

for those using sexed semen, are you still inseminating with it at 12 hours or are some of you inseminating with it laterthan that?

 

[Silver]

We do everything exactly the same as you would if you were using regular semen.

 

[Cowboy]

I have a few thoughts for everyone to consider -- I will try to be brief.

First off, the old standard of breeding 12 hours after you first see a cow standing is a Good and Bad deal. Case in point, every cow is different I agree, however, if you follow the time line of heat versus ovualtion, and then look into the time it takes semen to be able to fertilize after deposition, you will see the following --

Pre-estrous - 3-6 hours.
Standing estrous - 10-18 hours -- about 15 average believe it or not.
Post Estrous -- another 3 or more hours.

Time from deposition to be able to fertilize (Removal of cryo-protectants) will be about 3 hours give or take.

Total time to ovulation from -0- -- almost 30 hours.

Max time of life for frozen semen -- (Good semen) no more than 20 hours.

Sexed semen is severely stressed in the process of seperation, and is on the table alot longer than normal semen, therefore it will NOT last as long, and will NOT be as viable to begin with. Also, there are severely smaller numbers involved.

You REALLY need to be on time with this stuff or you will be very dissapointed. It will fertilize, don't get me wrong, but you just need to be on your A game from the start!

I hope that answers your wquestion -- borrom line is -- breed them later than you ever would with your 12 hour standard deal.

Personaly, I never touch a cow here -- A-I or a donor -- unless she has been completely out of heat for at least 6 hours, alot of itme more like 10! It works very well , but if I ever get ina  hurry, well there goes the conception rates!

Best of luck --

Terry

 

[bob b]

do you thaw at the same temperature for the same amount of time?

 

[Harwell Cattle]

Haven't used much sexed semen for normal breeding, but I have used quite a bit of sexed semen for flushes and we have had really good luck using two straws as close to 24 hrs as possible. 

 

[hereford]

The "viability" issue is not as much of an issue as people think it is with sexed semen. The sexed straws are subjected to the same post-thaw motility standards as conventional straws. They have to be motile just like conventional semen. The reason for the slightly lower conception rates is primarily because the straws are packed at a lower dose. The "quality" of a sexed straw compared to a conventional straw is much higher. Each sperm cell is selected one by one to go in to a sexed straw. Its just at a lower concentration.

You can use it in flushes. Its just that you need to be using the 5 million dose straw, as compared to the 2.1 million dose straw that is used solely for AI.

 

[hereford]

Also, we have good luck inseminating slightly later than we do with conventional semen. Because of the lower dose straw, the timing of ovulation is more critical. Thus the reason for the slightly later timing of insemination.

 

[Cowboy]

Every one re-read my above post -- my point exactly!

T I M I NG ---  T I M I N G  --- T I M I N G >>>>>>>>>>>>>>>>>>>>>> Conception

<cowboy>
Terry